Embryo Cryopreservation

What is Embryo Cryopreservation?

Embryo cryopreservation is a procedure of preserving fertilised egg(Embryo).

The development of and improvements in cryopreservation technology over the last decade have helped to increase overall clinical pregnancy rates from a single oocyte retrieved. There are two different types of cryopreservation: vitrification and slow freezing.

The process of slow freezing takes longer time and involves step-wise programmed decrease in temperature. The procedure is lengthy and hence, there are increased chances of ice crystal formation within the cell which in turn can have extremely deleterious effects

Vitrification is a rapid method of freezing over few minutes. It solidifies the cell into a glass-like state, thus, avoiding the formation of both intra and extracellular ice crystals and hence, risk of damage to cells. This is usually accomplished through the use of relatively high concentrations of cryoprotectant agents (CPA) and/or very high cooling rates (15,000–30,000°C/min). This technique is far more time-efficient, requiring only several minutes (12-15 mins.), compared with 1-2 hours with controlled-rate slow freezing.

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